general_pathology:infectious:infectious_diseases_155
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Which of the following is attached to the labelled antibody in IHC?
”Explanation”
The enzyme is attached to the labelled antibody. The other three molecules are substrates for the colour change.
In amplification IHC. Biotin is attached to the labelled antibody and then an enzyme labelled avidin or streptavidin is added to form a chain of labelled antibodies. This amplifies the signal
From: https://www.mblbio.com/bio/g/support/method/immunohistochemistry.html
From: https://www.bosterbio.com/protocol-and-troubleshooting/immunohistochemistry-ihc-principle?srsltid=AfmBOopxyXQiCuZQFIbKxBjxMgaPfl5KhJzJDn_QCbtTlkgJ9_U0MefL
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