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What are the steps in CRISPR gene editing?
Binding of guide RNA to a CRISPR-associated (Cas) gene, binding of gRNA to complementary sequence in genome, double strand DNA cleavage by cas nuclease, introduction of random mutations by DNA repair/introduction of target ds DNA
Binding of guide DNA (gDNA) to a CRISPR asociated (cas) gene, binding of cas-gDNA complex to homologous sequence in genome, cleavage of DNA by restriction endonucleases, DNA repair by a CRISPR associated (cas) enzyme introduces random mutations by non-homologous end joining/insertion of gene by homologous DNA repair
Binding of guide DNA (gDNA) to a CRISPR-associated (Cas) gene, binding of gRNA to complementary sequence in genome, double strand DNA cleavage by cas nuclease, introduction of random mutations by DNA repair/introduction of target ds DNA
Binding of guide RNA to complementary DNA sequence, cleavage of dsDNA by restriction enzyme, DNA repair by a CRISPR associated (cas) enzyme introduces random mutations by non-homologous end joining/insertion of gene by homologous DNA repair
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