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--- general_pathology:infectious:infectious_diseases_155 [2026/03/07 23:28] – created achamings
+++ general_pathology:infectious:infectious_diseases_155 [2026/03/07 23:38] (current) – achamings
@@ Line 4: / Line 4: @@
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-The other three molecules are substrates for the colour change.
+The enzyme is attached to the labelled antibody. DAB and Fast red are substrates for the colour change.
+In amplification IHC. Biotin is attached to the labelled antibody and then an enzyme labelled avidin or streptavidin is added to form a chain of labelled antibodies. This amplifies the signal
 {{ :general_pathology:infectious:ihc.png?direct |}}
 From: https://www.mblbio.com/bio/g/support/method/immunohistochemistry.html
+{{ :general_pathology:infectious:ihc2.png?direct |}}
+From: https://www.bosterbio.com/protocol-and-troubleshooting/immunohistochemistry-ihc-principle?srsltid=AfmBOopxyXQiCuZQFIbKxBjxMgaPfl5KhJzJDn_QCbtTlkgJ9_U0MefL
 </accordion-item>
 </accordion>